Veterinary Microbiology question bank-3
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veterinary Microbiology
Questions Part-1-Veterinary Microbiology question bank-3
1. Koch’s postulates was derived by using which bacterium ?
2. Kanagawa reaction is exhibited by…
3. Father of Microbiology
4. In presence of specific antibody, Streptococcus pneumoniae shows —— reaction .
5. Father of Bacteriology
6. Small pox vaccine was developed by ——–in the year1796.
7. A polymer of glycerol phosphate that is present only in G+ bacteria cell wall
8. Rabies vaccine was first done on
9. Loeffler and Frosch shares the credit of discovery of——
10. The only anti TB drug that has the ability to destroy the acid fastness of Mycobacterium
11. The bacteria that is used to evaluate the phenol coefficient using Rideal Walker method
12. Mastitis causing Str.agalactiae and Str. dysgalactiae are classified as group —– and group—- respectively as per Lancefield classification.
13. The substance present normally in spores at high levels,but decreases during the favourable condition.
14. Greyish-white medusa head type of colony is shown by Bacillus anthracis in which medium ?
15. Chinese letter arrangement and metachromatin granules are features of …
16. Agent that causes Summer Mastitis
17. Growth of E.rhusiopathiae is favoured by which aminoacid ?
18. Tuberculous lesions are prominent in digestive tract rather than in respiratory tract in … ?
19. Etiological agent of Calf Diphtheria
20. Characteristic features of abortion in cattle caused by B.abortus
21.Type of vaccines used against brucellosis in calves & cows.
22. Kennel Cough in dogs caused by … ?
23. Pasteurella, Yersinia and Listeria have one thing in common as part of their staining character.
24. Etiological agent of fowl coryza
25. Classification of Pasteurella species ?
26. The best medium for an enhanced growth of campylobacter
27. In Mc Konkeys agar, E.coli produces ——— colonies whereas Salmonella produces ——
28. Ringer and Gillespie medium is used for the growth of ….
29. ‘Symptomatic anthrax is the synonym for….
30. Para anthrax in pigs is caused by ….
31. Gaint cells of Langhans are absent in T.B affecting which species ?
32. In H&E staining T.B calcification appear as……. color.
33. Epitheloid cells fuse to form syncytia and it enters ……….stage in Johne’s diseases.
34. Among domestic species ………. is most susceptible to anthrax.
35. Condition in sheeps under 1year of age, affected by Cl.septicum due to toxaemia
36. Pulmonary Adenomatosis in sheeps by retrovirus is …….. whereas Cl.botulinum type D infection in cattle is …..
37.Dunkop and Dikkop are forms of …….
38 Diagnostic test for E.I.A
39. Inclusion bodies in Fowl pox is …… and in cow pox is……..
40. Instrument used to perform the Polymerase Chain Reaction
41. Ulcerative enteritis in poultry caused by ……..
42. Infectious encephalomyelitis caused by Flavi virus transmitted by ixodes ricinus.
43 Granules present within the Guarnieri body.
44.Synonym for Infectious bulbar paralysis caused by Herpes.
45. Two medium used for the growth of mycoplasma.
46. In McFaydean reaction ,color of organism and capsule ?
47. Bursitis in horse caused by Brucella abortus
48. The etiological agent of ‘Struck’ in sheep
49. Bottle brush appearance in Gelatin stab is growth feature of ………
50. Visna/Maedi in sheep is caused by………
Answers Part-1 :
Veterinary Microbiology question bank-3
1.Bacillus anthracis
2.Vibrio parahaemolyticum
3. Louis Pasteur
4. Quellung reaction
5. Robert Koch
6. Edward Jenner
7. Teichoic acid
8. Joseph Meister
9. FMD Virus
10. Izoniazid
11. Salmonella Typhi
12. B and C ; A is S. pyogenes
13. Calcium Dipicolinate
14. Nutrient Agar
15. Corynebacteria
16. Corynebacteria pyogenes
17. Tryptophan
18. Poultry
19. Fusobacterium Necrophorus
20. Necrotic placentitis and Leathery placenta
21. Strain 19 (living) and Strain 45/20(killed) respectively
22. Bordetella bronchiseptica
23. Bipolar staining
24. Haemophilus gallinarum
25. Robert’s and Carter’s serotyping
26. Thiol medium
27. Pink ; Colourless
28. Leptospira
29. Black Quarter
30. Clostridium septicum
31. Canines and Felines
32. Blue
33. Symplasma
34. Sheep
35. Braxy or Bradsot
36. Jaagsiekte ; Lamsiekte
37. African Horse Sickness
38. Coggin’s Test
39. Bollinger bodies ; Guarnieri bodies
40. Thermocycler
41. Clostridium colinum
42. Louping ill
43. Paschen’s granules
44. Psuedorabies/Mad Itch/Aujezky’s disease
45. PPLO Agar and Frey’s medium
46. Blue ; Pink
47. Poll Evil and Fistulous Withers
48. Clostridium perferinges Type C
49. Erysipelothrix rhusiopathiae and Clostridium perferinges
50. Retro virus
Questions Part-2Veterinary Microbiology question bank-3
1.In Streptococci chain formation is due to the cocci dividing in----------------------------------Plane.
2. Toxin is employed in diagnosis of Scarlet fever----------------------------------------.
3. Selective media for Streptococci is...........................................
4. Woman's curling hair type of growth is characteristic of---------------,
5. The extracellular toxins of B. anthracis are composed of--------,.-----,.--------and----------.
6. In anthrax-affected animals the spleen revealed, ---------------consistency.
7. -------------Phage is commonly used to identify anthrax bacillus.
8. Lancefield classification is mainly based on --------antigen of the cell wall.
9. The CAMP test is based on the partial haemolysis of the.--------- toxin of Staphylococci.
10. On Blood agar Staphylococcus releases V factor which favours the growth of ----------organism.
11. All Clostridia are motile except --------
12. All Clostridia are non capsulated except,----------
13. Selective media for Clostridia is-------------
14. Based on the growth in Robertson cooked meat media Clostridia classified into ---------and ----
15. Western duck sickness is caused by.--------
16. Cl. botulinum toxin produces characteristic_-------- appearance in mice.
17. Strangles is caused by.----------
18. Botryomycosis in horse is caused by---------------
19. Staphylococcus hyicus causes.--------------------------in pigs.
20. Selective media for staphylococci is----------------------------
21. Clostridium species----------and---------- produces stormy fermentation in litmus milk.
22. Malignant odema in cattle is caused by.-----------
23. Cl. chauvoel type B causes-------------in cattle.
24. Struck in sheep is caused by.-------------
25. Cl. welchi ferments lactose and produce acid clot in litmus milk is called as---------
26. Braxy in sheep is caused by---------
27. Bacillus anthracis capsule is rich in-------------
28. Sudan Black B stain is commonly used to demonstrate -----------of B.anthracsis.
29. In gelatin stab growth of B. anthracis resembles ------------------------appearance.
30. Clostridia produce acid and clot in, _----------medium.
31. gelatin stab culture, Cl. tetani produces.-------------type of growth.
32. Listeria exhibits characteristic -----------motility in 24hr broth culture, incubated at 25°C.
33. The haemolysin of L. monocytogenes is known as,--------------.
34. Neural form of listeriosis is called as--------------
35. Listeriosis abortion typically occurs in------------trimester of pregnancy.
36. Isolation of listeria requires a process called-------------
37. Swine erysipelas is caused by --------------
38. Luxurient growth of M. tuberculosis on LJ medium is known as,----------
39. The cell wall of the Mycobacteria composed of ----------,.-----------,.-----------and-------.
40. Haematogenou spread of tubercle results--------------------------- form of tuberculosis.
41. Urticarial form of erysipelas infection in pig is called as-------------------
42. In gelatin stab culture E. rhusiopathiae gives ----------type of growth.
43. Caseous lymphadenitis in sheep is caused by--------------------
44. To demonstrate Corynebacterium granules-------,.---------------and----------stains are commonly used.
45. All Corynebacteria are Urease positive except---------------
46. Two pathogenic species of Nocardia are----------------and---------------------
47. Bovine farcy is cause by.-----------------------
48. The cell walls of Mycobacteria are rich in--------------------------.
49.------------------is called as Timothy grass bacillus.
50. The acid-fast property of mycobacterium was discovered by----------------------.
Answers Part-2
Veterinary Microbiology question bank-3
One or Single Plane
Erythrogenic
Edwards Medium
Bacillus anthracis
Edema Factor, Protective Antigen & Lethal Factor
Black Cherry Jam
Cherry Gamma Phage
Carbohydrate Antigen or C-antigen
Beta
Haemophilus
Cl. perfringens
Cl. perfringens
Robertson's Cooked Meat Media
Saccharolytic & Proteolytic
Cl. botulinum
Wasp Waist
Streptococcus equi sub sps equi
Staphylococcus aureus
Exudative Epidermitis or Glassers disease
Mannitol Salt Agar
Cl. perfringens, Cl. septicum
Cl. septicum
Black leg
Cl. perfringens type c
Stormy Clot Fermentation
Cl. septicum
Poly-D-glutamate
Spores
Inverted fir tree
Litmus Milk
Fir tree
Tumbling
Listeriolysin
Circling Disease
2nd
Cold Enrichment
Erysipelothrix rhusiopathiae
Eugenic
Peptidoglycan, Arabinomannan, Mycolic acid
Milliary
Diamond Skin Disease
Bottle Brush Type
Corynebacterium pseudotuberculosis
Albert's neissers, ponder's stain
Corynebacterium bovis
Nocardia steroids, Nocardia farcinica
Nocardia farcinica
Mycolic acids
Mycobacterium phlei
Ehrlich, 1882
Questions Part-3Veterinary Microbiology question bank-3
VMC-111: General Veterinary Microbiology (1+1)
QUESTION BANK
Short notes
1. List the contributions of Louis Pasteur
2. List the differences between prokaryotes and eukaryotes
3. Write briefly about gram negative bacterial cell wall
4. Write briefly about bacterial capsule.
5. Describe briefly the bacterial growth curve.
6. Describe the chemiosmotic hyphothesis.
7. Embden – Meyerhof pathway of glycolysis
8. Describe the bacterial cell count methods
9. Describe transport of nutrients by group translocation.
10.Describe briefly about transduction.
11. Asexual fungal spores
12. Describe about recombination and reassortment in viruses.
13. Describe the Baltimore classification of viruses.
14. Describe the three domain classification of organisms.
15. Write about the antiviral action of interferon.
16. Write about the different types of cell cultures.
17. Describe the structure of flagella and bacterial motility.
18. Describe the grouping of bacteria with respect to optimum temperature.
VMC-111: General Veterinary Microbiology (1+1)
Essay
1. List the steps in virus replication and describe the virus transcription, genome replication
and assembly.
2. a. Describe the EMP pathway.
b. Write about the bacterial growth curve.
3. a.Describe the grouping of bacteria with respect to oxygen requirement.
b. Write about the contributions of Robert Koch.
4. Describe about transduction and conjugation in bacteria.
5. Describe methods used for measuring bacterial growth.
6. a. Write about plasmids.
b. Describe about bacterial mutation.
7. a. Lytic and lysogenic bacteriophage infections.
b. Compare bacteria and viruses.
8. a. Compare endotoxins and exotoxins.
b. Describe the arrangements in bacteria.
9. Describe composition of a virus and about the different symmetries found in viruses.
10. Write about the methods used to classify bacteria.
Questions Part-4Veterinary Microbiology question bank-3
Questions from Subject: Veterinary Microbiology
1. Koster stain used for............................?
2. Fungal cell wall doesn’t contain…………………………………?
3. Ag binding site on Ab by......................................................................?
4. Germinal center of lymph node mainly contains.........................?
5. In late fetal life which are the organs of haemopoises.....................?
6. Which Ab is present in maximum conc. in milk.................?
7. Which Ab is present during allergic reaction......................?
8. Pearl eye is associated with which disease..........................?
9. Substance added to enhance the immunity by using inactivated
microbes……....................?
10.Exotoxins are mainly secreted by..............................?
11.Leechy disease also known as...........................................?
12.Russell body is present in......................................?
13.Humoral immunity is associated with................................?
14.Which one is known as scavenger cells...........................?
15.Mycolic acid is present in cell wall of...............................................?
16.Lumpy jaw is caused by...............................?
17.Protein which is specific for viral infection.............................?
18.Strangles in horses is caused by....................................?
Answers Part-4
Veterinary Microbiology question bank-3
1. Brucella spp.
2. Muramic acid
3. Both heavy and light chain
4. B-cells
5. liver and spleen
6. Ig A
7. Ig E
8. Marek’s disease
9. Adjuvant
10. Gram+ve bacteria
11.Angara disease
12.Plasma cell
13.B lymphocytes
14.Macrophages
15.Mycobacterium spp.
16.Actinomycosis bovis
17.Interferon
18.Streptococcus equi
Questions Part-5Veterinary Microbiology question bank-3
1. Write short notes on any FIVE of the following:
(a) Write the contributions of Pasteur and Beijerink.
(b) Explain briefluy the structure and functions of bacterial cellwall.
(c) Briefly explain the concept of containment facility.
(d) Write a commonly used method for isolation of pure culture of bacterium.
(e) Distinguish between continuous and synchronous cultures.
(f) Distinguish between bacteria and mycoplasmas.
(g) Give a brief account of slime molds.
(h) Briefly explain the morphological features protozoa.
UNIT-1
(a) Discuss the kingdoms of organisms and phylogenetic tree.
Or
(b) Explain the importance of Beragy”s Mannual in bacterial taxonomy.
UNIT II (a) Write the special staining techniques for microscopic identification of
Microorganisms.
Or
(b) Explain the importance of selective, differential and maintenance
Microbiology.
UNIT III (a) Describe the reproduction and spore formation in bacteria.
Or
(b) Explain the biochemical methods for measurement of microbial growth.
UNIT IV
(a) Discuss the economic importance of fungi with examples.
(15)
Or
(b) Write on outline classification of algae by Fritsch.
Questions Part-6Veterinary Microbiology question bank-3
In Streptococci chain formation is due to the cocci dividing in _____________Plane.
Toxin is employed in diagnosis of Scarlet fever____________.
Selective media for Streptococci is________.
Woman’s curling hair type of growth is characteristic of __________.
The extracellular toxins of B. anthracis are composed of _______, ________ and _________.
In anthrax-affected animals the spleen revealed ________ consistency.
_______ Phage is commonly used to identify anthrax bacillus.
Lancefield classification is mainly based on antigen of the cell wall.
The CAMP test is based on the partial haemolysis of the ______ toxin of Staphylococci.
On Blood agar Staphylococcus releases V factor which favours the growth of ______ organism.
All Clostridia are motile except _________ .
All Clostridia are non capsulated except ________.
Selective media for Clostridia is ___________.
Based on the growth in Robertson cooked meat media Clostridia classified into______ and _______.
Western duck sickness is caused by _______________.
Cl. botulinum toxin produces characteristic__________ appearance in mice.
Strangles is caused by ________________.
Botryomycosis in horse is caused by _________.
Staphylococcus hyicus causes ____________ in pigs.
Selective media for staphylococci is _____________.
Clostridium species ________ and ________ produces stormy fermentation in litmus milk.
Malignant odema in cattle is caused by ____________.
Cl. chauvoei type B causes _________ in cattle.
Struck in sheep is caused by __________.
Cl. welchi ferments lactose and produce acid clot in litmus milk is called as_________.
Braxy in sheep is caused by _____________.
Bacillus anthracis capsule is rich in _______.
Sudan Black B stain is commonly used to demonstrate _________ of B. anthracsis.
In gelatin stab growth of B. anthracis resembles ________appearance.
Clostridia produce acid and clot in _______medium.
In gelatin stab culture, Cl. tetani produces __________ type of growth.
Listeria exhibits characteristic _______ motility in 24hr broth culture, incubated at 25°C.
The haemolysin of L. monocytogenes is known as _____________.
Neural form of listeriosis is called as ___________________.
Listeriosis abortion typically occurs in ____________ trimester of pregnancy.
Isolation of listeria requires a process called ________________.
Swine erysipelas is caused by __________ .
Luxurient growth of M. tuberculosis on LJ medium is known as ______.
The cell wall of the Mycobacteria composed of ______, ______ and ______ .
Haematogenous spread of tubercle results _______ form of tuberculosis.
Urticarial form of erysipelas infection in pig is called as___________________.
In gelatin stab culture E. rhusiopathiae gives _________ type of growth.
Caseous lymphadenitis in sheep is caused by __________.
To demonstrate Corynebacterium granules ________ and ________ stains are commonly used.
All Corynebacteria are Urease positive except ______________.
Two pathogenic species of Nocardia are ________ and __________.
Bovine farcy is cause by __________________.
The cell walls of Mycobacteria are rich in ______ .
________ is called as Timothy grass bacillus.
The acid-fast property of mycobacterium was discovered by ______________.
Write True or False
Streptococci are catalase and oxidase negative.
The capsule of Streptococci pyogenes is made up of polysaccharide.
When Streptococci treated with penicillin, spheroplast form will occur.
Beta haemolytic Streptococci produce complete haemolysis.
Griffith typing of Streptococci is mainly based on agglutination test.
Staphylococci are not able to grow in the media containing 7-10% NaCl.
S. aureus strains from dogs produce golden yellow colonies on nutrient agar.
Chemically the beta haemolysins of Staphylococci are phospholipase C.
Gangrenous mastitis in cattle is mainly due to alpha toxin of staphylococci.
Oxidase test is highly useful to differentiate Staphylococci and Streptococci.
Clostridium are anaerobic, spore forming, spindle shaped bacteria.
Clostridia are motile with monotrichous flagella.
Cl. odematiens is very strict anaerobe.
In Robertsons media proteolytic clostridium turns the meat pink colour and produce gas.
Soil is the natural habitat of Cl. tetani.
Cl. tetani is very potent invasive Clostridium.
Capsulated Cl. tetani giving the characteristic drum sticks appearance.
Cl. tetani is a non-capsulated and motile bacillus.
Stiff agar (3%) is commonly used to demonstrate swarming nature of Cl. tetani.
Streptolysin S is very potent cardiotoxic.
Staphylococci are catalase +ve and oxidase negative.
S. aureus causes bumble foot in poultry.
The word staphyle means twisted or coiled bacilli.
Tick pyaemia in 2-4weeks old lambs is caused by S. aureus.
Coagulative staphylococci are arranged in grapes like structure.
Cl. tetani is saccharolytic.
Cl. tetani spores are readily destroyed by 5% phenol or 0.1% mercuric chloride solution.
Cl. tetani serological type VI possesses peritrichous flagella.
Tetanolysin is a very potent neurotoxin.
Poultry are highly susceptible to tetanospasmin.
Ascending tetanus is more common in human and horses.
The species Cl. tetanomorphus and Cl. tetanoides produces drumstick spores as like Cl. tetani.
Administration of ATS within 4hrs of infection can prevent tetanus.
Botulinum toxins are very potent enterotoxins.
Botulism is mainly due to very rapid multiplication of bacteria at the site of infection and production of toxin.
Normal heat fixation of blood smears is sufficient to kill the B. anthracis bacilli.
B. anthracis spores are readily destroyed by 5% phenol.
In anthrax lethal toxin alone is responsible for production of local oedema and shock.
Poultry are highly resistant to anthrax.
Acute fatal form of anthrax is very common in pigs.
The Sterne vaccine contained spores of a non-capsulated a virulent mutant strain of B. anthracis.
Pulmonary anthrax is called as hide porter’s disease.
Anthracoids are motile, non-capsulated and non-spore formers.
The edges of the colonies of B. anthracis appear irregular and are known as medusa head colonies.
In bacillus, sporulation is inhibited by exposure to air.
The spores of Bacillus anthracis are wider than the vegetative form.
Bacillus species are catalase positive.
Listeria is motile at 37°C.
Listeria expresses flagellar protein only at higher temperature.
L. monocytogenes does not grow in media with 10% salt concentration.
Answers Part-6
Veterinary Microbiology question bank-3
1 One or Single Plane
2 Erythrogenic
3 Edwards Medium
4 Bacillus anthracis
5 Edema Factor, Protective Antigen & Lethal Factor
6 Black Cherry Jam
7 Cherry Gamma Phage
8 Carbohydrate Antigen or C-antigen
9 Beta
10 Haemophilus
11 Cl. perfringens
12 Cl. perfringens
13 Robertson’s Cooked Meat Media
14 Saccharolytic & Proteolytic
15 Cl. botulinum
16 Wasp Waist
17 Streptococcus equi sub sps equi
18 Staphylococcus aureus
19 Exudative Epidermitis or Glassers disease
20 Mannitol Salt Agar
21 Cl. perfringens, Cl. septicum
22 Cl. septicum
23 Black leg
24 Cl. perfringens type c
25 Stormy Clot Fermentation
26 Cl. septicum
27 Poly-D-glutamate
28 Spores
29 Inverted fir tree
30 Litmus Milk
31 Fir tree
32 Tumbling
33 Listeriolysin
34 Circling Disease
35 2nd
36 Cold Enrichment
37 Erysipelothrix rhusiopathiae
38 Eugenic
39 Peptidoglycan, Arabinomannan, Mycolic acid
40 Milliary
41 Diamond Skin Disease
42 Bottle Brush Type
43 Corynebacterium pseudotuberculosis
44 Albert’s neissers, ponder’s stain
45 Corynebacterium bovis
46 Nocardia steroids, Nocardia farcinica
47 Nocardia farcinica
48 Mycolic acids
49 Mycobacterium phlei
50 Ehrlich, 1882
Questions Part-7Veterinary Microbiology question bank-3
Herring bone appearance is seen in ………………. virus.
Viral enterotoxin is produced by ………………. virus.
Onion layer appearance of lymph node is seen in ……………….. disease.
Quellung reaction is due to the presence of ……………….. in bacteria.
Protein seen in between the envelope and capsid in virusus is called as ………………
Nipah virus belongs to ………………. family
As per the latest ICTV report (2018b) there are ……………. order, ………… family, …………. subfamily, …………. genus and …………… species of virus.
The serotype of FMD virus which was recently removed from vaccine in India is ……………………….
Strawberry lymph node is pigs is seen in ……………………….. disease.
Mixing vessel of influenza virus is ……………..
Blue eye in pigs is caused by ………………
Palisade arrangement is seen in …………….. bacteria.
Seagull wing appearance is seen in ……………… bacteria.
Tender coconut smell is characteristic to the colony of ……………. bacteria.
Pear shaped macroconidia are seen in …………………….. dermatophyte.
Eastern blot test is used to analyze ……………………………………………
Fungus which is abundantly seen in pigeon droppings is …………………………..
Pearl of string test is for ………………………… bacteria.
Germ tube test is used for ……………………………
Negative staining in electron microscopy is done by ……………………………..
Phenol in lactophenol cotton blue stain acts as …………………………………
Bipolar staining is seen in ………………………….., ………………………………….. and ………………………………….. bacteria.
Fluorescence seen in Woods lamp technique is due to the metabolite of ……………
Type of spore arrangement in hair shaft by dermatophyte can be …………………, ……………………. or ……………………
CIN agar is used for ……………………………… bacteria.
MUG test is based on the presence or absence of ………………………….. enzyme.
Magnetosomes are a kind of …………………. seen in some bacteria.
Erysipelas in humans is caused by ……………………………
Fish slime is a source of …………………….. bacteria.
Hjarres disease is caused by …………………………
Cup shaped depressions are seen on the surface of ………………….. virus.
Protein A is located on the walls of …………….. and binds to the Fc region of …………………… antibody.
Streptokinase activates …………..
The degree of the ability of a pathogen to cause disease is called as ……………
Exotoxins lose their toxicity when heated at …………………………… or treated with ……………….
Toxoids have ………………….. but no ………………….
………………………… of LPS is knows as endotoxin.
Lysozyme acts on the …………………. of bacteria.
Interferons are ……………… specific and ……………… non specific.
…………….. are the bacteriocins produced by E. coli
Primary immune response is predominated by ……………….. antibodies whereas secondary immune response is predominated by ………………… antibodies.
Single diffusion in one dimension is ……………………. test.
Double diffusion in one dimension is ……………………. test.
Double diffusion in two dimensions is ……………………..test.
……………………. Test is done to differentiate between recent infection and past infection.
The most commonly used reagent for the effective coating of soluble antigens on RBC is ……………………..
Membrane attack complex is ……………..
Side chain theory of antibody formation is proposed by …………………
The binding force between individual antibody combining site and the antigen determinant group is called as ……………….
J chain is seen in …………………. and ……………… immunoglobulins.
Answers Part-7
Veterinary Microbiology question bank-3
Paramyxovirus
Rotavirus
Caseous lymphadenitis
Capsule
Matrix
Paramyxoviridae
14 order, 150 family, 79 subfamily, 1010 genus and 5560 species
C
Swine fever
Pig
Porcine rubula virus
Corynebacterium
Campylobacter
Pasteurella
Microsporum nanum
Post-translational modifications of proteins
Cryptococcus neoformans
Bacillus anthracis
Candida albicans
Uranyl acetate
Fungicide
Pasteurella, Yersinia and Francisella
Tryptophan
Endothrix, Ectothrix and Favus
Yersinia
Beta-glucuronidase
Inclusion body
Streptococci
Erysipelothrix rhusiopathiae
E. coli
Calici
Staphylococcus aureus, IgG
Plasminogen
Virulence
60 to 80 °C, acid
Immunogenicity, Toxicity
Lipid A
Cell wall
Species, virus
Colicins
IgM, IgG
Oudin
Oakley-Fulthrope
Ouchterlony
Mercaptoethanol test
Tannic acid
C5b6789
Paul Ehrlich
Affinity
IgA and IgM
Questions Part-8Veterinary Microbiology question bank-3
BACTERIOLOGY AND MYCOLOGY With Answers
• The most commonly used endospore stain- Schaeffer-Fulton stain
• Capsule of B. anthracis is made of – D-glutamic acid
• Teichoic acid in Gram-positive bacterial cell wall has- Glycerol/ribitol
• Archaebacterial cell walls usually consists of – Proteins and polysaccharides
• Metachromatin granules serves as a reserve of – Inorganic phosphate
• The chemi-osmotic mechanism of ATP synthesis proposed by- Peter Mitchell
• Reducing media for anaerobic bacteria contains- Na-Thioglycolate
• Mycobacterium leprae is usually isolated in- Armadillos
• Selective media for S.typhi is – Bismuth sulphite agar
• Selective media for Staphylococcus aureus is- Mannitol Salt Agar
• Paraffin and mineral oils are often sterilized by- Hot air oven
• Sterilizing agent used in space crafts is- Ethylene oxide
• Test used to find the carcinogenicity of mutagens is- Ames test
• Three kingdom classification of bacteria proposed by- Carl Woere
• Pyrogen induced by endotoxins is – IL-1
• Lab test to detect the presence of endotoxins in preparations- Limulus assay
• Resoloving power of a compound microscope is – 0.2ì m
• Heat resistance of spores is due to presence of – Dipicolinic acid
• Target site of polymyxin is- Bacterial cell membrane
• Thickness of Gram+ve and Gram-ve cell walls are- 20-80nm and 2-7nm
respectively
• Most abundant membrane protein of Gram-ve bacterial outer membrane- Braun’s
lipoprotein
• Non-motile asexual sporangiospores of phycomycetes is – Aplanospores
• Exogenously borne sexual spores are known as – Basidiospores
• ‘Psuedohyphae is characteristic of – Candida albicans
• ‘North American blastomycosis’ is caused by- Blastomyces dermatitidis
• ‘European blastomycosis’ is caused by- Cryptococcus neoformans
• Asexual spores produced by fragmentation of hyphae is – Arthrospore
• Staining used for detection of fungi in pathological specimens- Periodic acid
Schiff stain
• Media used for stimulating production of chlamydospores of C. albicans- Corn
meal agar.
• Method used for observing the development of spores and hyphae of fungus is
called- Block-slide culture technique.
• Mucor is distinguished from other members of Phycomycetes by- Absence of
rhizoids
• Asexual spores of Ascomycetes are known as- Conidiospores
• ‘Bottle bacillus’ is the synonym for- Pityrosporum ovale
• ‘Pseudoglanders’ or ‘Japanese Farcy’ caused by Histoplasma capsulatum
• ‘Fluffy colonies’ , ‘Powdery colonies’ and ‘Lemon yellow colonies’ are produced
by- Microsporum distortum , M. gypseum and M. canis respectively.
• Most potent toxin producing aflatoxicosis is – B1
• ‘Facial eczema’ in cattle is caused by - Pithomyces chartarum
• “Farmer’s Lung” is caused by Micropolyspora faeni
• % of Agar used for the preparation of solid media is- 1.5%
• Microbial population can be maintained in the exponential growth and at constant
biomass for extended periods by using- Chemostat/Turbidostat
• The phenomenon by which bacteria monitor their own population density through
sensing the level of signal molecules- Quorum assay
• Indicator bacterium used in autoclaving is- Bacillus stearothermophilus (spores)
• The commonly used agent for ‘cold sterilization is - Gamma radiation
• The disinfectant action of chlorine is due to the formation of- Hypochlorous acid
• Linear chromosomal DNA is found in- Borrelia burgdoferi
• The topoisomerase that removes the supercoiling during replication- DNA gyrase.
• The negative stain used in electron microscopy is – Phosphotungstic acid
• Bacterium that exhibits directed swimming in response to Earth’s magnetic field
or to local magnetic fields- Aquaspirillum
• The structure that remain after the treatment of a gram –ve bacteria with
lysozyme/penicillin is – Spheroplast
• An inorganic solidifying agent used for autotrophic bacteria- Silica gel
• The procedure for staining the capsular material of B. anthracis is- MacFadyean
reaction.
• Botulism due to which type of toxin occurs in relation with phosphorous
deficiency- Type D
• Vole’s Bacillus is the synonym for- Mycobacterium murinum
• Specific media used for isolation of M. bovis is Stonebrink’s medium containing
Sodium pyruvate
• ‘Stormy clot’ reaction is characteristic of- Clostridium welchi
• The characteristic ‘earthy odour’ of the cultures is a feature of- Psuedomonas
psuedomallei
• ‘Flying sea gull’ appearance is characteristic of- Campylobacter
• Vaginal mucus agglutination test in cattle is employed for the diagnosis ofCampylobacter infection.
• Rolling disease in mice is caused by- Mycoplasma neurolyticum.
• Eaton’s agent is the synonym of- Mycoplasma pneumoniae
• Dick’s test is used for the id entification of- Streptococcus pyogens
• Chinese letter arrangement is seen for- Corynebacterium
• Fried lander’s bacillus is the synonym for Klebsiella pneumoniae
• Weil-Felix reaction is used for diagnosis of Rickettsial infections using Proteus
vulgaris X strain
• Swarming growth and fishy odour of colonies is characteristic of- Proteus
vulgaris
• Kauffman-White scheme is used for serotypic differentiation of Salmonella
• VR (Venkatraman-Ramakrishnan) medium is used as a transport media forVibrio cholerae
• ‘Epidemic typhus’ (Brill-Zinsser disease) is caused by Rickettsia prowazaki
• ‘Scrub typhus’ is caused by Rickettsia tsutsugamushi
• Rickettsial disease that are not arthropod borne are Q-fever and Trench fever
Questions Part-9Veterinary Microbiology question bank-3
BACTERIOLOGY AND MYCOLOGY With Answers
Fletcher’s media is used for cultivation of ………………………………
a. Borrelia
b. Leptospira
c. Salmonella
d. Listeria
ANS: b. Leptospira
For motility testing ……………………. Media is used
a. Liquid media
b. Semi solid media
c. Solid media
d. All the above
ANS: b. Semi solid media
Tryptic soy agar is a ……………………… media
a. Basal
b. Enriched
c. Selective
d. Enrichment
ANS: a. Basal
Concentration of salt in Mannitol salt agar is ………………………..
a. 2%
b. 6%
c. 3%
d. 5%
ANS: d. 5%
Egg containing media is used for the cultivation of ……………………………..
a. Mycobacteria
b. Mycoplasma
c. Chlamydia
d. Borrelia
ANS: a. Mycobacteria
Rhodamine will give …………………….. fluorescence
a. Apple green
b. Orange
c. Reddish
d. Bluish
ANS: c. Reddish
Mesosome is the invagination of ………………………..
a. Nuclear membrane
b. Plasma membrane
c. Cell wall
d. Capsule
ANS: b. Plasma membrane
Which among the following is having linear DNA?
a. Bordettela
b. Borrelia
c. Brucella
d. Blue green algae
ANS: b. Borrelia
Volutin granules are seen in ………………………….
a. Corynebacterium
b. Campylobacter
c. Clostridia
d. Listeria
ANS: a. Corynebacterium
…………………………….. is the numerically most abundant protein of gram –ve cell wall
a. Lipoprotein
b. Glycoprotein
c. Apoprotein
d. Flagellim
ANS: a. Lipoprotein
Flagellar antigen is …………………………
a. H antigen
b. O antigen
c. K antigen
d. F antigen
ANS: a. H antigen
Gram+ organisms have ………………… pair of flagellar rings
a. 1
b. 2
c. 3
d. 4
ANS: a. 1
Sheathed flagellum is seen in ……………………………….
a. Listeria
b. Leptospira
c. Salmonella
d. Leuconostoc
ANS: b. Leptospira
Which among the following shows pleomorphism?
a. Streptobacilli
b. Streptococci
c. Staphylococci
d. Lactobacilli
ANS: a. Streptobacilli
Quantitative ability of an organism to cause disease is ……………………………..
a. Virulence
b. Pathogenicity
c. Invasiveness
d. Adhesiveness
ANS: a. Virulence
……………………………………. Test is used to distinguish pathogenic Staphylococci
a. Catalase test
b. Coagulase test
c. CAMP test
d. MUG test
ANS: b. Coagulase test
…………………………….. is the indicator used in Citrate utilization test
a. Bromocresol purple
b. Bromothymol blue
c. Neutral red
d. Phenol red
ANS: b. Bromothymol blue
Sodium thaurochorate is a ……………………..
a. Bile salt
b. Buffer
c. Bile pigment
d. PH indicator
ANS: a. Bile salt
………………………………… pathway is used by Pseudomonas to oxidise aromatic compound to acetyl coA AND SUCCINATE
a. TCA cycle
b. Pyruvate decarboxylation pathway
c. Entner-Doudoroff pathway
d. Beta keto adipate pathway
ANS: d. Beta keto adipate pathway
Which among the following show bipolar staining characters?
a. Pasteurella and Salmonella
b. Pasteurella and Yersinia
c. Yersinia and Brucella
d. Yersinia and Salmonella
ANS: b. Pasteurella and Yersinia
Common laboratory contamionator is ………………………..
a. Pseudomonas aeruginosa
b. Protius vulgaris
c. Mycoplasma mycoides
d. Staphylococcus aureus
ANS: b. Protius vulgaris
Which among the following is true about Bacilllus anthracis?
a. Rough colony is pathogenic
b. Smooth colony is pathogenic
c. Mucoid colony is pathogenic
d. Both a & b
ANS: a. Rough colony is pathogenic
Type culture collections in India is maintained at …………………………
a. Ludhiana
b. Mukteshwar
b. Bareilly
c. Banglore
ANS: a. Ludhiana
……………………………… is a type specimen designated by the original discoverer of the species
a. Neotype
b. Holotype
c. Serotype
d. Biotype
ANS: b. Holotype
……………………………. Is a method of classification of bacteria using a number of parameters
a. Adansonian classification
b. Numerical classification
c. Polyphasic classification
d. Computer taxonomy
ANS: c. Polyphasic classification
Questions Part-10Veterinary Microbiology question bank-3
The ratio of diameter of objective lens to focal length is:
a. Numerical aperture
b. Resolution
c. Magnification
d. None of these
An example for positive eye piece:
a. Huygenian eyepiece
b. Ramsden eyepiece
c. Both a and b
d. None of these
________________ microscopy is used to determine dry mass of living cells and their nuclei
a. Darkfield microscopy
b. Laser microscopy
c. Interference microscopy
d. Flourescence microscopy
________________ types of specimens have to be used in electron microscopy
a. Hydrated specimen
b. Dehydrated specimen
c. Thick specimen
d. Stained specimen
Which among the following contain sterol in cytoplasmic membrane
a. Spirochetes
b. Mycoplasma
c. Clamydia
d. Clostridia
_________________ are also known as PPLO
a. Rickettsiae
b. Mycoplasma
c. Mycobacteria
d. Clamydia
________________ produces packet of 8 organisms or multiple of 8.
a. Neisseria
b. Sarcina
c. Veillonella
d. All the above
Normal fir tree appearence in gelatin stab medium is produced by
a. Bacillus anthracis
b. Clostridium
c. Erysipelothrix
d. Salmonella
Primary stain in gram staining is
a. Crystal violet
b. Methylene blue
c. Safranin
d. Carbol fuchsin
In Hucker staining method, gram positive cell appear ________________ in colour
a. Blue
b. Pink
c. Violet
d. Dark red
Which among the following is wrong about negative staining of bacteria?
a. Used to study cell shape
b. Can be done with eosin or nigrosin
c. Stain does not penetrate the cell
d. Heat fixation is required
Which among the following is acid fast positive?
a. Mycoplasma
b. Nocardia
c. Neisseria
d. Fusobacterium
Traunt staining fluorescence method is used for
a. Endoscope
b. Acid fast bacteria
c. Flagella
d. Gram positive bacteria
Popping test is done for the staining of
a. Exospore
b. Endospore
c. Pili
d. Flagella
Which among the following is used for endospore staining
a. Schaeffer-Fulton staining
b. Donner staining method
c. Burke staining method
d. Both a and b
Which among the following is used for flagellar staining?
a. Gray staining method
b. Leyson staining method
c. Dorner staining method
d. Both a and b
______________ is used in Ames test
a. Salmonella typhimurium
b. Shigella dysentriae
c. Staphylococcus aureus
d. Sreptococcus pneumoniae
Muller Hinton agar is used in
a. Ames test
b. Replica plating method
c. Antibiotic sensitivity test
d. Penicillin enrichment of mutants
Calcium chloride treated bacterial cells are competent for
a. Transduction
b. Transformation
c. Plasmid replication
d. Conjugation
Indicator in urea broth medium is _____________________
a. Phenol red
b. Bromocresol purple
c. Methyl red
d. Bromothymol blue
IMViC reaction for Yersinia is
a. + + – –
b. – + – +
c. – – + +
d. – + – –
Indole production can be detected by _____________________
a. Barritt’s reagent
b. Kovac’s reagent
c. Collin’s reagent
d. Baeyer’s reagent
Which among the following shows mixed acid fermentation of glucose
a. Shigella
b. Klebsiella
c. Enterobacter
d. Serratia
Which among the following is used as cryoprotectants in the storage of bacterial culture at -700C?
a. 10% glycerol
b. 10% DMSO
c. 15% glycerol
d. 15% DMSO
Voges proskeur test is based on the ability of organism to produce ________________ in glucose phosphate broth
a. Urea
b. Pyruvic acid
c. Acetoin
d. Phenylene diamine
Answers Part-10
Veterinary Microbiology question bank-3
1. a 2. b 3. c 4. b 5. b 6. b 7. b 8. b 9. a 10. c 11. d 12. b 13. b 14. b 15. d 16. d 17. a 18. c 19. b 20. a 21. d 22. b 23. a 24. a 25. c
Questions Part-11Veterinary Microbiology question bank-3
With Answers
MIASMATIC THEORY was proposed by ……………………………………….
a. Themisson
b. Girolamo Frocaistoro
c. Thassalus
d. Hippocrates
Ans : d. Hippocrates
CONTAGION THEORY was proposed by ……………………………………….
a. Girolamo Frocaistro
b. Anton Von Leeuwenhoek
c. Athanisius Kircher
d. Francesco Redi
Ans : a. Girolamo Frocaistro
Louis Pasteur developed vaccines against the following diseases except …………………………
a. Anthrax
b. Fowl cholera
c. Tuberculosis
d. Rabies
Ans : Tuberculosis
The first person to use a solid medium for cultivating bacteria …………………………………………….
a. Louis Pasteur
b. Francesco Redi
c. Robert Koch
d. Theoder Schwann
Ans : c. Robert Koch
IVRI was established as Imperial Bacteriological laboratory in ……………………………………………………………
a. Pune ,1988
b. Muktheswar,1980
c. Pune, 1980
d. Muktheswar,1980
Ans: a. Pune,1988
Organisms lacking a rigid cell wall are included under …………………………………………………………….
a. Gracilicutes
b. Firmicutes
c. Mendosicutes
d. Tenericutes
Ans: d. Tenericutes
Gram positive bacterial cellwall in addition to peptidoglycan contains …………………………………………..
a. Mycolic acid
b. Teichioc acid
c. Muramic acid
d. Pimilic acid
Ans : b. Teichoic acid
Limkage between N-acetyl glycosamine and N- cetyl muramic acid is …………………………………………….
a. beta 1,4
b. beta 1,3
c. beta 1,2
d. beta 1,5
Ans : a. beta 1,4
Capsule and Slime layer are together known as …………………………………………
a. Endoplasmin
b. Glycocalyx
c. Exotoxin
d. Somatic antigen
Ans : b. Glycocalyx
Based on the arrangement of flagella Pseudomaonas aeuruginosa is …………………………………….
a. Peritrichous
b. Amphitrichous
c. Lophotrichous
d. Monotrichous
Ans: c. Lophotrichous
Which among the cocci organism have/has flagella?
a. Sporocercina
b. Rhodococcus
c. Planococcus
d. All the above
Ans: d. all the above
Impermeability of spore is due to ……………………………………………….
a. Poly-D-Glutamic acid
b. Calcium Dipicolinic acid
c. Thecoic acid
d. Diamino Pimilic acid
Ans: b. Calcium Dipicolinic acid
Which among the following is a diplococci?
a. Micrococci
b. Neisseria
c. Streptococci
d. Rhodococci
Ans: b. Neisseria
Feulgen staining is used to stain …………………………… which appears ………………………….. in colour.
a. Nucleoid, purple
b. Cytoplasm, blue
c. Ribosome, purple
d. volutin granules, red
Ans : a. Nucleoid, purple
Cooked meat media can be used for the culture of …………………………………..
a. Anaerobic
b. Capnophilic
c. Mesophilic
d. Thermophilic
Ans: a. Anaerobic
The method used to preserve bacteria in powder form is …………………………………..
a. Tyndallisation
b. Lyophilization
c. Deep freezing
d. Cryopreservation
Ans: b. Lyophilization
Refractive index of Canada Balsam is ………………………………..
a. 1.535
b. 1.524
c. 1.483
d. 1.460
Ans: a. 1.535
……………………………………………………… are produced by gram positive bacteria where there is complete absence of cell wall.
a. L-form
b. Spheroplast
c. Protoplast
d. Mycoplasma
Ans: c. Protoplast
Fractional sterilization was devised by ………………………………………….
a. Louis Pasteur
b. John Tyndall
c. John Snow
d. Joseph Lister
Ans: b. John Tyndall
Seitz Filter is used for filtering ………………………………………
a. Culture broth
b. Serum
c. Toxin from culture
d. Viral growth medium
Ans: b. Serum
Preservative used for serum is ………………………………..
a. Merthiolate
b. HCHO
c. 70% alcohol
d. 10% alcohol
Ans: a. Merthiolate
Garrod test is used ………………………………….
a. to evaluate virulence of bacteria
b. to check phenol coefficient
c. to check antibiotic susceptibility
d. to estimate D-value
Ans: b. to check phenol coefficient
Anaerobes cannot withstand oxygen due to lack of ………………………………….
a. Superoxide dismutase
b. Catalase
c. Peroxidase
d. both a and b
Ans: d. both a and b
Piezophiles are bacteria which can withstand great ……………………………….
a. atmospheric pressure
b. osmotic pressure
c. radiation
d. temperature
Ans: a. atmospheric pressure
Carbohydrates are added to the bacterial media at a rate of …………………………….
a. 0.5 – 2 %
b. 4 – 5 %
c. not added
d. 7%
Ans: a. 0.5 – 2 %
Questions Part-12Veterinary Microbiology question bank-3
The first viral vaccine to be produced in cell culture…………….
First vaccine that proved to protect against cancer ……………….
The antigenic drift refers to ……………… in influenza virus while antigenic shift refers to…………….
The highest taxon introduced for virus taxonomy in ICTV 10th report…………..
Virus cultured in embryonated chicken eggs by intravenous route……………..
Bollinger body inclusion bodies are observed in……………………
The type C of FMD virus discontinued in vaccination in India has not be recorded since………….
Avian nephritis virus is inoculated in embryonated chicken eggs by …………… route.
Delaware, Georgia, Massachusetts, Connecticut are the strain of virus infecting birds called…………
Rabies vaccine given by Louis Pasteur on 6th of July was derived from spinal cord ………. animal.
Ritonavir antiviral used against HIV prevents virus infectious cycle by interfering with enzyme………
Post weaning multisystemic wasting syndrome in pigs is caused due to……………
The virus responsible for bladder neoplasia in cattle also associated with bracken fern poisoning is………………
Duck plague- duck viral enteritis virus belongs to family……………..
The viral outbreak in Assam in the year 2020 first time reported in India was of……………
The virus using T structure for replication initiation in host……………
The shepherd’s crook shape is of…………….
FMD virus was discovered by…………………..
Receptor binding site of Coronavirus is present in ………………..
String of pearl structure is present in…………….
High pathogenic avian influenza virus have intravenous index more than…….
Inactivating agent used for FMD vaccine is…………………
Vector responsible for transmission of African swine fever virus …………………..
Bjorn Sigurdsson type of infection is seen in virus…………………
Nipah virus causing zoonotic infection in humans has animal host………………..
Japanese encephalitis causing neuroinvasive disease is transmitted from animals to humans by vector…………………………..
SA-14-14-2 the live attenuated vaccine is used against………………….
Monkey fever a tick borne haemorrhagic fever is the common name of the viral zoonotic disease…………………
Crimean Congo haemorrhagic fever the viral zoonotic disease transmitted by ticks belong to family……………………….
Natural host of Ebola virus is…………………….
Fowl paralysis and red leg syndrome are observed in birds affected with…………………
Pseudolumpy skin disease virus of bovine belongs to the family……………………….
The countries where terrestrial rabies has been controlled the transmission could take place from…………………
FMD infection persists in ruminants for long period and is localized in site…………………..
Scrapie disease of sheep is caused by ……………………
Answers Part -11
Poliovirus vaccine
Marek’s disease vaccine
Recombination, reassortment
Realm
Bluetongue virus
Fowl pox virus
1995
Yolk sac
Infectious bronchitis
Rabbit
protease
Porcine circovirus
Papillomavirus
Herpesviridae
African swine fever virus
Parvovirus
Ebola virus
Loffler and Frosch
S protein
Circovirus
1.2
Binary ethylene amine
Ornithodorus, soft tick
Visna-maedi, Lentivirus
Pig
Culex tritaeniorhynchus
JE disease
Kyasanur Forest Disease
Bunyaviridae
Fruit bats
Marek’s disease
Herpesviridae
vampire bats
Pharynx
prions
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